An ELISA (enzyme-linked immunosorbent assay) is a plate-based assay for detecting and quantifying soluble molecules such as antibodies, proteins, peptides, haptens and drugs.
In an ELISA, the antigen (molecule to be detected and quantified) is immobilized onto a solid surface (microplate) and then complexed with an antibody that is conjugated to a reporter enzyme (e.g., Horseradish Peroxidase). These complexes are then incubated with the appropriate substrate for the reporter enzyme to produce a measurable product. The most essential element of any ELISA is a highly specific antibody-antigen interaction.
ELISA Applications
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- Medical Diagnostics: Detection of infectious diseases and autoimmune disorders by identifying specific antibodies or antigens in samples.
- Food Industry: Ensures food safety by testing for allergens and pathogens, like peanuts and E. coli, in food products.
- Environmental Monitoring: Screens for environmental contaminants in water and air, including pesticides and allergens.
- Research and Development: Biological research by measuring hormone levels and investigating protein interactions in disease studies.
- Veterinary Diagnostics: Diagnosing animal diseases and managing breeding by testing for specific diseases and hormone levels.
Browse Available Product Categories Below
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| Immunoglobulin | Biomarkers | ELISA Reagents and Consumables | Custom Assay Development |
Custom ELISA Development
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If you are looking to develop your own ELISA but find the process challenging, our assay development branch, CytoGroup, is here to assist. Specializing in the development of biosensors and diagnostic tools, CytoGroup offers comprehensive services to transform your concept into a fully functional commercial product. Our team of experts will guide you through every stage of development, from design to manufacturing, ensuring your assay meets the highest standards of quality and performance. Learn more about how CytoGroup can assist in your ELISA assay development.
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CytoGroup Inc. offers high-quality and fully optimized ELISA kits that allow you to reliably and consistently measure target-specific antibodies with confidence. In addition, services such as: lot reservation, bulk packaging and custom target development are available to provide convenience to assist with your research needs.
Download: ELISA Product Brochure
Download: ELISA Service Brochure