Product overview:
Co-developed with Cytodiagnostics.
The Human Thrombin solid-phase sandwich ELASA (Enzyme-linked Aptasorbent Assay) is designed to quantify the target analyte “sandwiched” between a capture antibody and a detection aptamer.
The microplate wells supplied with the kit are pre-coated with a target-specific antibody. Upon adding the samples, standards, or controls, the target is captured by the immobilized antibody and subsequently binds to the next added detector aptamer.
A secondary enzyme conjugate binds the target-attached detector aptamer and a substrate solution reacts with the enzyme-antibody-target complex to develop a measurable colored end-product which correlates to the concentration of target analyte present in the original sample. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Our ELASA kit has several benefits:
- Ready to use. Simply add standards and samples directly to wells. No need for lengthy blocking or rehydration steps.
- High sensitivity, specificity and reproducibility.
- Fully validated in the sample types listed below.
- 96-well plate is breakable into 12 x 8 well strips.
Download: ELISA Service Brochure
Product Specification
| Protein name | Thrombin |
| Alternate names | Coagulation factor II, F2, alpha-thrombin |
| Species reactivity | Human |
| Assay format | Solid-phase Sandwich ELASA (quantitative) |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Sample volume | 100 μL |
| Assay length | 2 hrs |
| Analytical sensitivity | <1.4 ng/mL |
| Assay range | 2.5 – 160 ng/mL |
| Intra-assay CV | <6% |
| Inter-assay CV | <12% |
| Recovery (%) | Serum: 101.4, Plasma: 93.3, Cell culture media: 109.8 |
| Detection & Instrument | Colorimetric, Microplate Reader |
| UniProt ID | (Human) P00734 |
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